The best way to initiate mushroom fruiting is to periodically introduce fresh air exchange (FAE) once full substrate colonization has occurred. FAE, along with light and humidity, is one of the primary pinning triggers. It’s important to understand that mushrooms are not photosynthetic, but photosensitive — meaning they respond to light direction rather than its intensity. A light source strong enough to read a book is ideal. Less is more when it comes to lighting; aggressive lighting can dry out the substrate and damage your grow block.
If you’re using our isolated spore syringes, the genetics are designed for optimal performance in sealed bag environments. These strains are bred to thrive in conditions with higher CO₂ levels, which is common in bag grows. Unlike mono tubs or plastic bins, bag tek is a modern method that reduces contamination risk while requiring patience for traits like CO₂ resistance to be expressed. Due to reduced FAE in bags, you can expect mushroom fruiting to begin a week or two later than traditional methods.
When Should I Start Inducing Mushroom Fruiting?
Once your grain and substrate have incubated for 7–10 days and appear fully colonized, begin fresh air introduction. Break up the grain as much as possible to speed colonization and accelerate fruiting conditions. Spray the inside of the grow bag 3–4 times with tap water. At this point, contamination is less likely because the mycelium has developed its own immune defense.

How Much Fresh Air Exchange (FAE) is Needed For Mushroom Fruiting?
FAE plays a critical role in mushroom fruiting. If you live in a humid region (above 70% ambient humidity), you may not need to spray or open the bag at all. Humidity inside the grow bag will naturally hover around 90% or higher — ideal for fruiting. If you’re in a dry environment (like California or Arizona), open the bag every 2–3 days and lightly mist the inside. Never let water pool at the bottom of the bag — spray sparingly and maintain the seal with a resealable clip.
How Much Light is Necessary?
For effective mushroom fruiting, 6–12 hours of indirect sunlight or artificial lighting per day is enough. Office lighting, white LED strips, or placement near a bright window will suffice. Continuous 24-hour lighting works but isn’t necessarily better.
Popular strains like Golden Teacher, B+, Blue Meanie, and Inca Stargazer are particularly forgiving and will usually produce full canopies under modest conditions.
Signs Your Mushroom Grow Kit is Fruiting
- Rhizomorphic (thread-like) mycelium strands extending from the substrate
- Beaded water droplets on the surface
- Visible white pins or dots (primordia) forming, often on the sides or bottom of the bag if light penetrates
To avoid side pins, secure the bag with a rubber band and use a top-down light source.
Strains such as True Albino Teacher, Albino Penis Envy, and other albino varieties may take 3–4 weeks to fruit. Advanced strains like Enigma or Bluey Vuitton may take 5 weeks or more. These longer fruiting timelines often correlate with higher potency — strains that take longer typically test above 1% alkaloid content.
Going for a Second Flush?
Remove all mushrooms and aborts after the first flush. Take the entire cake out of the bag and place it on your counter for cleaning. The risk of contamination only becomes significant after multiple flushes, as the substrate’s pH drops and becomes more acidic (around 4–5). Any leftover pins can rot and lead to bacterial blotch, so a thorough harvest is critical.
Dunking for a Second Flush
After cleaning the cake, rinse it under gentle water and let a slow stream soak it completely. Then return it to the bag and reseal. Second and third flushes can sometimes yield even better results. Expect to wait about a week for the next wave of fruiting.
Toward the end of the cycle, your substrate block will feel light, dry, and spongy — typically weighing around 1.5 lbs. That’s when you know it’s finished.